Mail host on .env laravel doesn't change Laravel 5.1

I launched sudo nohup php artisan queue:work --daemon on my server with laravel 5.1, and now I have change MAIL_HOST in my .env, (before I had "mail.mysitem.com" and now I put my intern ip example : 192.168.1.212 ) but it still send mail with old hostname, how I can apply change on .env?

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